mimetic poly i c  (InvivoGen)

Journal: Molecular oral microbiology

Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

doi: 10.1111/omi.12270

Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in type I interferon expression by RT-qPCR. Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test. All data analysis was performed using GraphPad Prism version 8.2.0 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com.

Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test, Software

Journal: Molecular oral microbiology

Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

doi: 10.1111/omi.12270

Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in interferon-stimulated gene (ISG) expression by RT-qPCR. Measured genes were divided into each ISG basal expression group, according to Table 2: Low (A), Medium (B), and High (C). Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test.

Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test